amidase: meaning, definition, pronunciation and examples

Very Low (Specialist)
UK/ˈæmɪdeɪz/US/ˈæmɪdeɪs/ (also /ˈæmɪdeɪz/)

Technical/Scientific

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Quick answer

What does “amidase” mean?

An enzyme that catalyzes the hydrolysis of amides, releasing ammonia and a carboxylic acid.

Audio

Pronunciation

Definition

Meaning and Definition

An enzyme that catalyzes the hydrolysis of amides, releasing ammonia and a carboxylic acid.

Any enzyme belonging to a specific class (EC 3.5.1.4) that acts on the carbon-nitrogen bond in linear amides, often involved in metabolic pathways for nitrogen-containing compounds.

Dialectal Variation

British vs American Usage

Differences

No significant lexical or conceptual differences. Spelling conventions (e.g., 'catalyses' vs. 'catalyzes') may apply in surrounding text, not the term itself.

Connotations

Purely technical, neutral in both varieties.

Frequency

Exclusively used in biochemical, microbiological, and related research contexts with equal rarity in both varieties.

Grammar

How to Use “amidase” in a Sentence

The amidase [hydrolyses/cleaves] the amide bond.Amidase activity was [measured/detected/observed] in the extract.

Vocabulary

Collocations

strong
enzymehydrolysisamidesubstrateactivity
medium
bacterialrecombinantpurifiedcatalyzesspecific
weak
highassaygenepathwaycloning

Examples

Examples of “amidase” in a Sentence

verb

British English

  • The enzyme amidases the substrate efficiently.
  • This pathway involves amidasing the compound.

American English

  • The enzyme amidases the substrate efficiently.
  • This pathway involves amidasing the compound.

adverb

British English

  • The reaction proceeded amidase-dependently.
  • The protein was cleaved amidase-specifically.

American English

  • The reaction proceeded amidase-dependently.
  • The protein was cleaved amidase-specifically.

adjective

British English

  • The amidase activity was localised to the cytoplasm.
  • An amidase-deficient mutant was created.

American English

  • The amidase activity was localized to the cytoplasm.
  • An amidase-deficient mutant was created.

Usage

Meaning in Context

Business

Virtually never used.

Academic

Exclusively used in biochemistry, molecular biology, and microbiology research papers and textbooks.

Everyday

Never used.

Technical

Core term within its specific biochemical niche for describing enzyme function and metabolic engineering.

Vocabulary

Synonyms of “amidase”

Strong

EC 3.5.1.4

Neutral

amide hydrolase

Weak

deamidase (context-dependent, often less specific)

Vocabulary

Antonyms of “amidase”

amidase inhibitorsynthetase (anabolic enzyme)

Watch out

Common Mistakes When Using “amidase”

  • Misspelling as 'amidaze' or 'amidise'.
  • Incorrect plural: 'amidases' (correct) not 'amidasees'.
  • Confusing it with 'amylase' (starch-digesting enzyme).

FAQ

Frequently Asked Questions

No, it is a highly specialized technical term used almost exclusively in biochemistry and related fields.

In specialist jargon, it is occasionally verbalised (e.g., 'The enzyme amidases the substrate'), but the noun form is vastly more common.

Both are hydrolases. Amidases act on simple amide bonds (R-CO-NH2), while proteases act on peptide bonds (the amide bonds linking amino acids in proteins). All proteases are amidases, but not all amidases are proteases.

In British English, /ˈæmɪdeɪz/. In American English, /ˈæmɪdeɪs/ is common, though the /z/ ending is also heard. The stress is always on the first syllable: AM-i-dase.

An enzyme that catalyzes the hydrolysis of amides, releasing ammonia and a carboxylic acid.

Amidase is usually technical/scientific in register.

Phrases

Idioms & Phrases

  • None. The term is non-idiomatic.

Learning

Memory Aids

Mnemonic

Think: 'AMIde' + '-ASE' (enzyme suffix). It's the 'ase' (enzyme) that acts on an 'amide'.

Conceptual Metaphor

SCISSORS FOR A SPECIFIC KNOT: The enzyme is a precise cutting tool designed to sever the specific 'knot' of the amide bond.

Practice

Quiz

Fill in the gap
The gene encoding the was cloned and expressed in E. coli to study its kinetics.
Multiple Choice

What is the primary function of an amidase?